Kiely Allen Clearwater Third Appearance (1-5-0)
- Finished eighth at the 2021 Women's Senior Amateur Championship
- Earned a top-20 finish at the 2020 Florida Women's Senior Open
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Tama Caldabaugh Ponte Vedra Beach Fifth Appearance (4-6-2)
- Advanced to the Round of 16 at 2021 U.S. Senior Women's Amateur Championship
- Finished third at the 2021 Women's Senior Four-Ball Championship with Susie Fonde
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Melanie Clegg Merritt Island First Appearance
- Finished runner-up at the 2021 Women's Net Championship
- Captured the win at the 2020 Women's Net Championship
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Carolyn Creekmore Ponte Vedra Beach Second Appearance (1-1-1)
- Qualified for the 2021 U.S. Women's Senior Amateur Championship
- Finished inside the top-10 at the 2021 Women's Senior Amateur Championship
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- Advanced to the Round of 16 at the 2021 U.S. Women's Mid-Amateur Championship
- Finished sixth at the 2021 Women's Amateur Stroke Play Championship
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Lin Culver Palm Coast Fourth Appearance (3-4-2)
- Took a share of second place at the 2021 Women's Senior Amateur Championship
- Advanced to the Round of 64 at the 2021 U.S. Senior Women's Amateur
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- Finished tied for sixth at the 2021 Women's Mid-Amateur Championship
- Competed in the Junior Florida Cup as a junior golfer
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- Finished runner-up in the Middle Division at the 2021 Women's Mid-Amateur
- Took a fifth-place finish in the Senior Field at the 2020 Women's Senior Amateur
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Karen Fisher Palm Coast First Appearance
- Took a share of the 2021 Women's Shoot-Out title with Lin Culver
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Prudence Hornberger Clermont First Appearance
- Finished tied for 11th in the Middle Division at the 2021 Women's Mid-Amateur
- Earned a third-place finish in the Senior Field at the 2021 Women's Senior Amateur
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Susan Keane Orlando Second Appearance (0-2-1)
- Finished tied for 12th at the 2021 Women's Senior Amateur Championship
- Advanced to the Round of 16 at the Women's Amateur Senior Match Play
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Christine Martin Palm Harbor Fourth Appearance (3-5-1)
- Earned the win in the Middle First Division at the 2021 Women's International Four-Ball
- Finished fourth in the Middle Division at the 2021 Women's Mid-Amateur
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Sarah Matin Winter Garden Fourth Appearance (2-7-0)
- Qualified for the 2021 Women's Mid-Amateur Championship
- Earned a top-10 finish at the 2020 Women's Mid-Amateur Championship
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Kelli Pry Naples Second Appearance (2-1-0)
- Earned a share of sixth at the 2021 Women's Mid-Amateur Championship
- Qualified for the 2021 U.S. Women's Mid-Amateur Championship
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- Qualified for the 2021 Women's Senior Amateur Championship
- Captured the 2021 Women's Senior Amateur Match Play Championship
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Jodi Sullivan Saint Petersburg Second Appearance (1-2-0)
- Finished runner-up at the 2021 Women's Senior Four-Ball with Kim Keyer-Scott
- Finished tied for 26th at the 2021 Women's Senior Four-Ball Championship
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